BlastChip: Decoding Early Embryonic Development Through Microfluidics

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Early human embryonic development is a complex process characterized by precise cellular interactions and environmental cues. The blastocyst stage, marked by the formation of the inner cell mass (ICM) and trophectoderm (TE), is pivotal for subsequent embryonic development and implantation. While mechanical factors are increasingly recognized as influencing cell fate determination, their role in early embryogenesis remains largely unexplored. Human embryo research is fraught with ethical and practical challenges due to stringent regulations and limited availability. To circumvent these limitations, this project leverages the potential of blastoids, derived from pluripotent stem cells, as ethical and controllable models of early human development. These blastoids recapitulate key aspects of the blastocyst, including the formation of ICM- and TE-like structures. By integrating advanced microfluidic technologies, computational modeling, and biomaterials, this research aims to elucidate the impact and role of mechanical cues on blastocyst development. Through the development of microfluidic platforms capable of generating and culturing blastoids under controlled conditions, I will investigate the effects of mechanical forces on their growth, cell fate and maturation. Additionally, by encapsulating blastoids in zona pellucida mimicking hydrogels, we will explore the role of this unique mechanical environment on early embryonic development. This research has the potential to significantly advance our understanding of early human embryogenesis, with implications for improving in-vitro fertilization techniques and developing novel therapeutic strategies for infertility and other reproductive disorders.