BLM WY Amphibian Batrachochytrium Dendrobaditis PCR Testing and DNA Collection

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A. Project background information The BLM currently manages habitat for 3 amphibian species which are on the BLM sensitive species list: northern leopard frog (Rana pipiens), great basin spadefoot toad (Scaphiopus intermontanus), and the boreal toad (Bufo boreas boreas). All 3 of these amphibian species have experienced declines throughout their range due to a number of factors. Prevalence of disease (especially chytrid fungus (Bactrachochytrium dendrobaditis or B.d.)) has caused large die-offs of the boreal toad and northern leopard frog. The BLM is interested in understanding the extent of chytrid fungus infections across the field office. We are also interested in collecting DNA information on species? populations that will allow us to understand population dynamics and facilitate management of these species across their range in Wyoming. B. Project objective The project will involve performing PCR analysis of amphibian swab samples for B.d.. The project will also involve cataloguing and storing amphibian DNA for future conservation biology studies. C.Period of Project: 3 years

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