BD FACSDiscover A8 Cell Analyser with BD CellView Image Technology

contract award notice

Closed

DTU will like to buy a BD FACSDiscover A8 5 Laser 78 detectors from BD Biosciences. The instrument will include the following : • Forward Scatter: Label-free brightfield imaging channel that measures light scattered at small forward angles by the cell as it passes through the laser interrogation point. • SSC Imaging: Label-free imaging channel measuring light scattered at 90° perpendicular to the laser path, capturing internal cellular complexity. • Lightloss Imaging: Measures the total photons lost from the laser beam due to both scattering and absorption by the cell, allowing Label-free brightfield imaging morphological assessment. • Channel 1: Fluorescence imaging (530/30nm range) • Channel 2: Fluorescence imaging (600/60nm range) • Channel 3: Fluorescence imaging (788/225nm range) These imaging channels enable spatial analysis of protein localisation, cellular interactions, and morphological characteristics that remain invisible to traditional flow cytometry. The system generates quantitative image parameters including: • Area and Perimeter measurements for size discrimination • Aspect Ratio and Eccentricity for shape analysis • Diffusivity for texture characterisation • Correlation and Distance for multi-channel co-localisation • Radial Moment for signal distribution analysis. Applications enabled by this technology include: • Identification of subcellular protein localisation (nuclear vs cytoplasmic) • Detection of cell-cell interactions and immunological synapses • Discrimination of budding yeast cells and cell aggregates • Analysis of phagocytosis and particle uptake • Label-free morphological study for downstream sorting. Complementing the imaging capabilities, the instrument should hold a technology for full-spectrum flow cytometry. This system features: • 5 laser configuration: 349 nm (UV), 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-Green), 637 nm (Red) • 78 fluorescence detectors capturing complete emission spectra • 8 scatter and imaging detectors • System-aware spectral unmixing algorithms that adapt to instrument performance in real-time • Capability for 40+ parameter experiments with optimal resolution and sensitivity Complementing the imaging capabilities, the instruments should include a technology for full-spectrum flow cytometry. This system features: • 5 laser configuration: 349 nm (UV), 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-Green), 637 nm (Red) • 78 fluorescence detectors capturing complete emission spectra • 8 scatter and imaging detectors • System-aware spectral unmixing algorithms that adapt to instrument performance in real-time • Capability for 40+ parameter experiments with optimal resolution and sensitivity

Laboratory, optical and precision equipments (excl. glasses), Laboratory, optical and precision equipments (excl. glasses), Laboratory, optical and precision equipments (excl. glasses), Laboratory, optical and precision equipments (excl. glasses)

Denmark

TED Europa

Tender

Nuclear, Education